Journal: MicrobiologyOpen

Article Title: Combining in vitro and in vivo screening to identify efficient Pseudomonas biocontrol strains against the phytopathogenic bacterium Ralstonia solanacearum

doi: 10.1002/mbo3.1283

Figure Lengend Snippet: Ralstonia solanacearum strains used in these experiments, and their geographical origin

Article Snippet: Eight fluorescent Pseudomonad strains (CHA0, Pf‐5, Q2‐87, Q8R1‐96, 1M1‐96, MVP1‐4, F113, and Ph11C2) which have been studied extensively previously in biocontrol (Hu et al., ), and seven Ralstonia solanacearum strains (#1–#7), which were isolated as a part of annual river sampling survey in England and Wales by Fera Science Ltd., were used in the experiments (listed in Tables and ).

Techniques:

Journal: MicrobiologyOpen

Article Title: Combining in vitro and in vivo screening to identify efficient Pseudomonas biocontrol strains against the phytopathogenic bacterium Ralstonia solanacearum

doi: 10.1002/mbo3.1283

Figure Lengend Snippet: Experimental design quantifying direct inhibition of Ralstonia solanacearum strain #1 by four types of Pseudomonas strains using soft agar overlay assay

Article Snippet: Eight fluorescent Pseudomonad strains (CHA0, Pf‐5, Q2‐87, Q8R1‐96, 1M1‐96, MVP1‐4, F113, and Ph11C2) which have been studied extensively previously in biocontrol (Hu et al., ), and seven Ralstonia solanacearum strains (#1–#7), which were isolated as a part of annual river sampling survey in England and Wales by Fera Science Ltd., were used in the experiments (listed in Tables and ).

Techniques: Inhibition, Overlay Assay

Journal: MicrobiologyOpen

Article Title: Combining in vitro and in vivo screening to identify efficient Pseudomonas biocontrol strains against the phytopathogenic bacterium Ralstonia solanacearum

doi: 10.1002/mbo3.1283

Figure Lengend Snippet: Direct and indirect inhibition of Ralstonia solanacearum by Pseudomonas strains. (a) Direct inhibition on soft agar assays, where each interaction represents a different Pseudomonas strain and the average diameter of the inhibition zones (mm) against different R. solanacearum strains after 96 h growth on soft agar lawns ( N = 3). (b) Indirect inhibition of R. solanacearum by Pseudomonas strains' supernatant‐LB mixes (set up in 96‐well plates containing a 50:50 ratio of SN and 100% LB broth). The shade of red represents the growth reduction in R. solanacearum densities compared to the control (100% LB broth) treatment after 72 h of growth ( N = 3)

Article Snippet: Eight fluorescent Pseudomonad strains (CHA0, Pf‐5, Q2‐87, Q8R1‐96, 1M1‐96, MVP1‐4, F113, and Ph11C2) which have been studied extensively previously in biocontrol (Hu et al., ), and seven Ralstonia solanacearum strains (#1–#7), which were isolated as a part of annual river sampling survey in England and Wales by Fera Science Ltd., were used in the experiments (listed in Tables and ).

Techniques: Inhibition, Control

Journal: MicrobiologyOpen

Article Title: Combining in vitro and in vivo screening to identify efficient Pseudomonas biocontrol strains against the phytopathogenic bacterium Ralstonia solanacearum

doi: 10.1002/mbo3.1283

Figure Lengend Snippet: Testing the inhibitory effects of identified Pseudomonas secondary metabolites on Ralstonia solanacearum growth. Panel (a) shows the growth of seven R. solanacearum strains (in different panels) in various DAPG concentrations over time (1000 μM—red, 500 μM—blue, 100 μM—green, 50 μM—purple, and 0 μM LB broth [0 μM—control]—black). Panel (b) shows R. solanacearum strain #1 growth in the absence and presence of 100 μM of orfamides A and B. Panel (c) shows the growth of R. solanacearum strains #1 and #7 in the absence and presence of 100 μM of pyoluteorin. In all panels, bars show the standard error of the mean (±1 SEM) based on three replicates

Article Snippet: Eight fluorescent Pseudomonad strains (CHA0, Pf‐5, Q2‐87, Q8R1‐96, 1M1‐96, MVP1‐4, F113, and Ph11C2) which have been studied extensively previously in biocontrol (Hu et al., ), and seven Ralstonia solanacearum strains (#1–#7), which were isolated as a part of annual river sampling survey in England and Wales by Fera Science Ltd., were used in the experiments (listed in Tables and ).

Techniques: Control

Journal: MicrobiologyOpen

Article Title: Combining in vitro and in vivo screening to identify efficient Pseudomonas biocontrol strains against the phytopathogenic bacterium Ralstonia solanacearum

doi: 10.1002/mbo3.1283

Figure Lengend Snippet: Testing the inhibitory effects of orfamide variants isolated from Pseudomonas CHA0 strain on Ralstonia solanacearum growth. The black lines denote R. solanacearum density in the absence of the orfamide variants over time (control), while colored lines show the growth in the presence of orfamide variants isolated from CHA0. Orfamide variants “A” and “B” refer to two different fractions isolated separately during the extraction process. All error bars show the standard error of the mean (±1 SEM) based on 3 replicates.

Article Snippet: Eight fluorescent Pseudomonad strains (CHA0, Pf‐5, Q2‐87, Q8R1‐96, 1M1‐96, MVP1‐4, F113, and Ph11C2) which have been studied extensively previously in biocontrol (Hu et al., ), and seven Ralstonia solanacearum strains (#1–#7), which were isolated as a part of annual river sampling survey in England and Wales by Fera Science Ltd., were used in the experiments (listed in Tables and ).

Techniques: Isolation, Control, Extraction

Journal: MicrobiologyOpen

Article Title: Combining in vitro and in vivo screening to identify efficient Pseudomonas biocontrol strains against the phytopathogenic bacterium Ralstonia solanacearum

doi: 10.1002/mbo3.1283

Figure Lengend Snippet: Tomato plant infections by Ralstonia solanacearum strains #1 and #7 in the absence and presence of Pseudomonas strains. Panel (a) displays the percentage of wilted plants (mean wilting incidence) and panel (b) the mean aboveground dry weight of individual plants at the end of the experiment for all treatments. All error bars show the standard error of the mean (±1 SEM) based on three replicates (every replicate consisting of nine plants)

Article Snippet: Eight fluorescent Pseudomonad strains (CHA0, Pf‐5, Q2‐87, Q8R1‐96, 1M1‐96, MVP1‐4, F113, and Ph11C2) which have been studied extensively previously in biocontrol (Hu et al., ), and seven Ralstonia solanacearum strains (#1–#7), which were isolated as a part of annual river sampling survey in England and Wales by Fera Science Ltd., were used in the experiments (listed in Tables and ).

Techniques: